Rnai ( rna interference ) is a process by which double - stranded rna ( dsrna ) specifically suppresses the expression of a target mrna . since fire et . al Rnai ( rnainterference )是雙鏈rna介導(dǎo)的、序列特異的同源基因轉(zhuǎn)錄后的沉默效應(yīng)。
American andrew fire and craig mello are winners for the nobel prize in physiology or medicine for their discovery of rna interference - gene silencing by double - stranded rna 今年的諾貝爾生理學(xué)或醫(yī)學(xué)獎(jiǎng)授予美國(guó)人安德魯?法爾和克雷格?梅洛,以表彰他們發(fā)現(xiàn)了rna (核糖核酸)干擾機(jī)制。
Though wheellike in cross section , rotavirus is actually a three - layered sphere containing 11 segments of double - stranded rna , each of which consists of a single gene encoding a protein 雖然輪狀病毒的橫切面呈車輪狀,實(shí)際上其球面擁有三層構(gòu)造,中間含有11段雙股rna ,每段含有一個(gè)攜帶某蛋白質(zhì)編碼的基因。
Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis . the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation , 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain . referred to the published sequence two primers were designed and synthesized 給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株, 48小時(shí)后采取法氏囊,用雙licl分級(jí)沉淀法提純?nèi)L(zhǎng)基因組dsrna ,設(shè)計(jì)一對(duì)引物,通過(guò)rt - pcr方法進(jìn)行了體外擴(kuò)增,獲得hb株a節(jié)段基因全長(zhǎng)cdna 。
double-stranded rnaとは意味:《生化學(xué)》二本鎖{にほんさ}RNA double-stranded rna meaning:[Medicine] RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing ...